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Xenograft Mouse Models for Pancreatic Cancer
Alfa Cytology is a leading preclinical research service provider. We have extensive experience constructing transplantation models for pancreatic cancer (PC). Based on our comprehensive expertise in this area and advanced technology platform, we can develop various PC xenograft models for our customers for research and preclinical purposes.
Overview of Transplantation Models
Transplantation models involve the transplantation of mouse or human cells/spheres/organoids/tissues into recipient mice. Compared to genetically engineered mouse models (GEMMs), transplantation models are easier to handle and have a relatively low and more predictable tumor latency. Transplantation models can be classified as in situ implantation models (in the pancreas) or ectopic implantation models (subcutaneous, intraperitoneal, intravenous, intrapleural, or intracardiac) depending on the location of cell implantation.
Fig. 1 Generation of allograft and xenograft transplantation models. (Jana, R., et al., 2023)
Moreover, according to the transplant source, transplantation models can be divided into syngeneic (allograft) and xenogeneic (xenograft). The allograft models are established by transplanting mouse-derived tumor cells and tumors into mice. In contrast to the allograft model, xenografts require the implantation of human tumors or cancer cells into immunocompromised mice, and PC cell lines or spheroids serve as common sources for transplantation.
Our Services
Over the past decades, xenograft models have been the backbone of cancer research, providing an effective environment for studying and evaluating novel therapies. We can construct different types of xenograft models for PC research. Several xenograft models of PC are available for our customers to choose from.
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Besides, we also offer a complete suite of laboratory services, including but not limited to the following.
- Transplantation study design
- Selection of appropriate cell line/cancer model
- Host animal selection
- Subcutaneous or orthotopic xenografts
Why Choose Us?
Scientific Experience
Professional team of scientists and more than ten years of experience in pancreatic cancer
Customized Service
Tailored services dedicated to ensuring customer satisfaction
Data Security
Strictly keep confidential the client's project information and experimental data
Quick Reply
Our customer service representatives are available 24 hours a day from Monday to Sunday
Case Study - Bxpc-3 Xenograft Model in NPG Mice
- Model Introduction
The BxPC-3 human pancreatic cancer xenograft model provides a validated preclinical platform for studying pancreatic adenocarcinoma growth and therapeutic response. The model is established through subcutaneous or orthotopic implantation of BxPC-3 cells into immunodeficient NPG mice, enabling robust tumor development and quantitative assessment of treatment efficacy.
- Model Information
- Model: Bxpc-3 Xenograft Model
- Animals: NPG (NOD-Prkdcem26Cd52Il2rgem26Cd22NjuCrl) Immunodeficient Mice
- Age: 6-8 Weeks
Cancer Type: Adenocarcinoma
- Weight: 18-22 g
- Model Construction
The model was established via subcutaneous or orthotopic implantation of human BxPC-3 pancreatic cancer cells into NPG mice. For orthotopic implantation, a suspension of 1×106 cells in 40 µL of Matrigel-enhanced medium is injected directly into the pancreas, enabling anatomically relevant tumor development and progression. Resulting tumors are monitored by caliper twice weekly.
Fig. 2 Workflow of Bxpc-3 xenograft model establishment. (Source: Alfa Cytology)
- Model Data
- Subcutaneous Implantation: Tumors show progressive growth post-inoculation, with clear separation between control and treatment groups (e.g., T-cell vs. CAR-T cell therapy) observable within the experimental timeline.
- Orthotopic Implantation: Tumor take rate exceeds 90%, with consistent tumor progression in the pancreatic microenvironment. The model is suitable for survival studies and metastasis observation.
Fig. 3 In vivo fluorescence images and tumor growth curves of pancreatic cancer BxPC-3 cells transplanted orthotopically. (n=6). *p < 0.05, **p< 0.01. Data are presented as mean ± standard error (SEM). (Source: Alfa Cytology)
Xenograft models serve as powerful research tools. CDXs are widely used in preclinical studies because of their low cost, rapid and efficient drug screening, and simple tumor size assessment. The xenograft models are significantly improved by establishing PDXs, thus bypassing the need for cancer cells to adapt to cell culture conditions. If you are interested in our services, please contact us for more details. You can contact our staff directly and receive professional, reliable, and fast feedback.
Reference
- Jana, R., et al.; Mice as an Experimental Model to Understand the Pathobiology of Diseases. 2023. DOI:10.5772/intechopen.1001835.
